CST, 84510S, CPS1/Hep Par-1 Antibody

Polyclonal Antibody for studying CPS1. Validated for Western Blotting. Highly specific and rigorously validated in-house, CPS1/Hep Par-1 Antibody (CST #84510) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity CPS1/Hep Par-1 Antibody recognizes endogenous levels of total CPS1/Hep Par-1 protein. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human CPS1/Hep Par-1 protein. Antibodies are purified by peptide affinity chromatography. Background Carbamoyl phosphate synthetase 1 (CPS1), a rate-limiting enzyme in the urea cycle, catalyzes the conversion of ammonia and bicarbonate to carbamoyl phosphate in mitochondria. Non-small cell lung carcinoma (NSCLC) cells with oncogenic and loss of the tumor suppressor LKB1 express CPS1 and depend on this enzyme for growth. CPS1 maintains pyrimidine/purine balance in these cancer cells. Silencing CPS1 reduces the pyrimidine to purine ratio and stalls DNA synthesis, leading to DNA damage and cancer cell death (1). In addition, the tumor suppressor p53 represses the expression of urea cycle enzymes CPS1, OTC, and ARG1 and, therefore, causes the accumulation of ammonia, suppressing cancer growth (2). Furthermore, research studies suggest that hypermethylation-mediated downregulation of expression may contribute to the progression of normal hepatocytes to hepatocellular carcinoma (HCC) (3). CPS1 was identified to be the antigen detected by Hep Par-1 antibody (4). Alternate Names carbamoyl-phosphate synthase (ammonia); Carbamoyl-phosphate synthase [ammonia], mitochondrial; carbamoyl-phosphate synthase 1; carbamoyl-phosphate synthase 1, mitochondrial; carbamoyl-phosphate synthetase 1, mitochondrial; Carbamoyl-phosphate synthetase I; carbamoylphosphate synthetase I; CPS1; CPSase I; CPSASE1; CPSM; PHN Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 165 SOURCE: Rabbit