CST, 84546S, CXXC5 (D1O4P) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CXXC5. Validated for Western Blotting,Immunoprecipitation,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, CXXC5 (D1O4P) Rabbit Monoclonal Antibody (CST #84546) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Immunofluorescence (Immunocytochemistry): 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity CXXC5 (D1O4P) Rabbit Monoclonal Antibody recognizes endogenous levels of total CXXC5 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu172 of human CXXC5 protein. Background The CXXC-type zinc finger protein 5 (CXXC5) is a nuclear protein that regulates gene expression and is involved in the regulation of cell growth and differentiation, apoptosis, cell adhesion, and cytoskeletal organization. The CXXC5 protein is also known as retinoid-inducible nuclear factor (RINF) as it was originally identified from a set of genes upregulated by retinoic acid stimulation (1). CXXC5 is a transcriptional activator of the vascular endothelial growth factor receptor VEGFR2. The CXXC5 protein regulates differentiation and migration of endothelial cells and subsequent blood vessel formation downstream of bone morphogenic protein (BMP) signaling (2). CXXC5 also regulates TNFα-induced apoptosis by facilitating phosphorylation of Smad3 and the nuclear translocation of Smad4 (3). Expression of CXXC5 in skeletal muscle regulates expression of genes involved in skeletal myogenesis (4). This nuclear factor also plays an important role in the regulation of normal myelopoiesis. The gene is localized to the 5q31.2 chromosomal region that is often involved in abnormalities associated with various myeloid malignancies, and CXXC5 over-expression is associated with decreased overall survival in human AML (5). Interestingly, CXXC5 is also over-expressed in many solid tumors, and high expression is also correlated with poor prognosis in breast cancer (6). Alternate Names CF5; CXXC finger 5; CXXC finger 5 protein; CXXC finger protein 5; CXXC-type zinc finger protein 5; CXXC5; HSPC195; Putative MAPK-activating protein PM08; Putative NF-kappa-B-activating protein 102; Q7LFL8; retinoid-inducible nuclear factor; RINF; WID; WT1-induced Inhibitor of Dishevelled Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 33 Source/Isotype: Rabbit IgG