CST, 8459T, Sall4 (D16H12) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying SALL4. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, Sall4 (D16H12) Rabbit Monoclonal Antibody (CST #8459) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunofluorescence (Immunocytochemistry): 1:100 - 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 86590 . Protocol Available protocols: Western Blotting, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity Sall4 (D16H12) Rabbit Monoclonal Antibody recognizes endogenous levels of total Sall4A and Sall4B proteins. Western blot analysis detects additional bands that are likely Sall4 isoforms. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala311 of human Sall4 protein. Background Members of the gene family encode putative zinc finger transcription factors highly expressed during development (1). Sall4 is expressed very early in development with other pluripotency regulators, such as Oct-4 and Nanog (2). Recent studies suggest Sall4 works as a master regulator that controls its own expression and the expression of Oct-4 in a transcriptional regulation feedback loop governing stem cell pluripotency and stem cell fate (2,3). Immunohistochemical studies indicate that Sall4 is a sensitive and specific diagnostic marker for primary germ cell tumors and yolk sac tumors (4,5). Research studies have shown that Sall4 is constitutively expressed in acute myeloid leukemia (AML) and is a probable effector of the Wnt/β-catenin signaling pathway in this disease (6). In addition, mutations in have been implicated in human malformation syndromes, including Duane-radial ray syndrome (Okihiro syndrome) and Acro-renal-ocular syndrome (7). Alternate Names dJ1112F19.1; DRRS; HSAL4; MGC133050; sal-like 4 (Drosophila); Sal-like protein 4; SALL4; spalt like transcription factor 4; Zinc finger protein 797; Zinc finger protein SALL4; ZNF797 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 80, 142 Source/Isotype: Rabbit IgG