CST, 84618S, IL-1 alpha Antibody

Polyclonal Antibody for studying IL1 alpha. Validated for Western Blotting. Highly specific and rigorously validated in-house, IL-1 alpha Antibody (CST #84618) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity IL-1 alpha Antibody recognizes endogenous levels of total IL-1α protein. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human IL-1α protein. Antibodies are purified by peptide affinity chromatography. Background Interleukin 1 alpha (IL-1α) belongs to the IL-1 family of cytokines with 11 members including IL-1β. IL-1α is expressed in many cell types of both hematopoietic and non-hematopoietic origins under steady state, and its expression can be increased in response to appropriate stimuli (1,2). Like IL-1β, IL-1α is also synthesized as a precursor (pro-IL-1α) and can be cleaved into smaller mature forms. However, both pro-IL-1α and the cleaved form of IL-1α are biologically active and can activate the signaling pathway through the membrane receptor IL-1R1. IL-1α is active both as a secreted form and as a membrane-bound form. Due to such characteristics, passive leakage of IL-1α from dying cells can activate inflammation, leading some researchers to consider IL-1α as a key "alarmin in the cell" that alerts the host to damage or injury (3,4). In addition, IL-1α can also enter the nucleus to modulate transcription (5,6). Alternate Names Hematopoietin-1; IL-1 alpha; IL-1A; IL1; IL1-ALPHA; IL1A; IL1F1; interleukin 1 alpha; interleukin 1, alpha; Interleukin-1 alpha; preinterleukin 1 alpha; pro-interleukin-1-alpha Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 30 SOURCE: Rabbit