CST, 84654SF, SHANK3 (D5K6R) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying SHANK3. Validated for Western Blotting,Immunofluorescence (Frozen). Highly specific and rigorously validated in-house, SHANK3 (D5K6R) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #84654) is ready to ship. Product Usage Information This product is the carrier free version of product #64555. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 64555 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity SHANK3 (D5K6R) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total SHANK3 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ile1210 of human SHANK3 protein. Background The SHANK family proteins, also known as proline-rich synapse-associated proteins, consist of SHANK1, SHANK2, and SHANK3. SHANK proteins act as scaffolds at the neuronal post-synaptic density (PSD) (1), where they play a critical role in PSD assembly of excitatory synapses during development (2). While recruitment of SHANK proteins to the synapse is independent of their interaction with Homer (3), proper synaptic targeting of SHANK1 is mediated by interactions between its PDZ domain and PSD proteins (4). At the synapse, SHANK proteins interact with NMDA receptors and metabotropic glutamate receptor complexes (5). Research studies have proposed the involvement of SHANK proteins in autism and neurodegenerative diseases (1). Alternate Names DEL22q13.3; KIAA1650; proline rich synapse associated protein 2; Proline-rich synapse-associated protein 2; ProSAP2; PSAP2; SCZD15; SH3 and multiple ankyrin repeat domains 3; SH3 and multiple ankyrin repeat domains protein 3; SHAN3; shank postsynaptic density protein; SHANK3; shank3 postsynaptic density protein; SPANK-2 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 220 Source/Isotype: Rabbit IgG