CST, 8466S, Phospho-GSK-3 beta (Ser9) (D85E12) Rabbit Monoclonal Antibody (PE Conjugate)

Monoclonal Antibody for studying GSK3-beta (Ser9) phosphate. Validated for Flow Cytometry (Fixed/Permeabilized). Highly specific and rigorously validated in-house, Phospho-GSK-3 beta (Ser9) (D85E12) Rabbit Monoclonal Antibody (PE Conjugate) (CST #8466) is ready to ship. Product Usage Information Flow Cytometry (Fixed/Permeabilized): 1:50 Storage Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibodies. Protect from light. Do not freeze. Protocol Available protocols: Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity Phospho-GSK-3 beta (Ser9) (D85E12) Rabbit Monoclonal Antibody (PE Conjugate) detects endogenous levels of GSK-3β only when phosphorylated at Ser9. This antibody conjugate reacts with denatured components of bovine serum, including BSA. Species Reactivity: Human, Mouse, Rat, Hamster, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser9 of human GSK-3β protein. Background Glycogen synthase kinase-3 (GSK-3) was initially identified as an enzyme that regulates glycogen synthesis in response to insulin (1). GSK-3 is a ubiquitously expressed serine/threonine protein kinase that phosphorylates and inactivates glycogen synthase. GSK-3 is a critical downstream element of the PI3K/Akt cell survival pathway whose activity can be inhibited by Akt-mediated phosphorylation at Ser21 of GSK-3α and Ser9 of GSK-3β (2,3). GSK-3 has been implicated in the regulation of cell fate in and is a component of the Wnt signaling pathway required for and mammalian development (4). GSK-3 has been shown to regulate cyclin D1 proteolysis and subcellular localization (5). Alternate Names glycogen synthase kinase 3 beta; Glycogen synthase kinase-3 beta; GSK-3 beta; GSK3B; GSK3beta; GSK3beta isoform; Serine/threonine-protein kinase GSK3B Specification REACTIVITY: H M R Hm Mk SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG