CST, 8467S, Phospho-Zyxin (Ser142/143) (D1E8) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying zyxin (Ser142/Ser143) phosphate. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-Zyxin (Ser142/143) (D1E8) Rabbit Monoclonal Antibody (CST #8467) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Phospho-Zyxin (Ser142/143) (D1E8) Rabbit Monoclonal Antibody recognizes endogenous levels of zyxin protein only when phosphorylated at Ser142 and Ser143. Species Reactivity: Human, Mouse, Rat, Monkey, Dog Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser142 and Ser143 of human zyxin protein. Background The zyxin family of proteins includes LIMD1, ajuba, trip6 and zyxin, each of which contains three LIM domains at the carboxy-terminus. Zyxin family members associate with the actin cytoskeleton and are components of both the cell-cell junction adhesive complex and the integrin-mediated adhesive complex. They shuttle in and out of the nucleus where they may function in transcriptional activation (1). Zyxin is involved in the regulation of mechanical force-induced actin polymerization at focal adhesions (2), and in regulation of adhesion and migration, possibly through recruitment of Ena/VASP proteins to focal adhesions (3). Zyxin interacts with and may regulate the function of the tumor suppressor myopodin, which inhibits tumor growth and metastasis (4). Alternate Names ESP-2; HED-2; ZYX; Zyxin; Zyxin-2 Specification REACTIVITY: H M R Mk Dg SENSITIVITY: Endogenous MW (kDa): 78 Source/Isotype: Rabbit IgG