CST, 84706S, CHMP2A Antibody

Polyclonal Antibody for studying CHMP2A. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, CHMP2A Antibody (CST #84706) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody . Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity CHMP2A Antibody recognizes endogenous levels of total CHMP2A protein. Species Reactivity: Human, Mouse, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human CHMP2A protein. Antibodies are purified by peptide affinity chromatography. Background CHMP2A is a component of ESCRT-III (endosomal sorting complex required for transport-III) (1,2). The ESCRT system is comprised of multiple protein complexes denoted as ESCRT-0, -I, -II, and -III, and VPS4 ATPase that are recruited to various cellular membranes and function fission reactions (3,4). Yeast contains four core ESCRT-III subunits: Vps20, Snf7, Vps24, and Vps2. Humans share these subunits and additionally have four Snf7 paralogs (CHMP4A-D) and two Vps2 paralogs (CHMP2A and B). The ESCRT-III complex comprising CHMP2A is involved in various membrane events, including multivesicular bodies, viral budding, cytokinesis, nuclear membrane reformation, and phagophore closure during autophagy and mitophagy (5-9). Alternate Names BC-2; BC2; Charged multivesicular body protein 2a; CHM2A; CHMP2; CHMP2A; chromatin modifying protein 2A; Chromatin-modifying protein 2a; hVps2-1; putative breast adenocarcinoma marker (32kD); Putative breast adenocarcinoma marker BC-2; Vacuolar protein sorting-associated protein 2-1; VPS2; VPS2 homolog A; Vps2-1; VPS2A Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 28, 31 SOURCE: Rabbit