CST, 84800S, Stat2 (D9J7L) Rabbit Monoclonal Antibody (InTraSeq™ 3' Conjugate 3093)

Monoclonal Antibody for studying Stat2. Validated for Single Cell Analysis - InTraSeq™ 3'. Highly specific and rigorously validated in-house, Stat2 (D9J7L) Rabbit Monoclonal Antibody (InTraSeq^™ 3' Conjugate 3093) (CST #84800) is ready to ship. Storage Supplied in PBS (pH 7.2), 2 mM EDTA, 0.05% Triton X-100, 2 mg/mL BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody . Protocol Available protocols: Single Cell Analysis - InTraSeq™ 3' Specificity / Sensitivity Stat2 (D9J7L) Rabbit mAb (InTraSeq™ 3' Conjugate 3093) recognizes endogenous levels of total Stat2 protein. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu706 of human Stat2 protein. Background Stat2 (113 kDa), originally purified from the nuclei of alpha-interferon-treated cells, is critical to the transcriptional responses induced by type I interferons, IFN-alpha/beta (1,2). Knockout mice with a targeted disruption of Stat2 have higher susceptibility to viral infection and altered responses to type I interferons (3). Stat2 is rapidly activated by phosphorylation at Tyr690 in response to stimulation by IFN-alpha/beta via associations with receptor-bound Jak kinases (4). Unlike other Stat proteins, Stat2 does not form homodimers. Instead, activated Stat2 forms a heterodimer with Stat1 and translocates to the nucleus. There, it associates with the DNA-binding protein p48 and forms the transcriptional activator complex, interferon-stimulated gene factor 3 (ISGF3), promoting transcription from the ISRE (5). Alternate Names IMD44; interferon alpha induced transcriptional activator; ISGF-3; MGC59816; p113; PTORCH3; Signal transducer and activator of transcription 2; signal transducer and activator of transcription 2, 113kDa; STAT113; STAT2 Specification REACTIVITY: H M SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG