CST, 84835T, NKG7 (E6S2A) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying NKG7. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry),Flow Cytometry (Fixed/Permeabilized). Available in 2 sizes. Highly specific and rigorously validated in-house, NKG7 (E6S2A) Rabbit Monoclonal Antibody (CST #84835) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunofluorescence (Immunocytochemistry): 1:100 - 1:200 Flow Cytometry (Fixed/Permeabilized): 1:50 - 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody . For a carrier free (BSA and azide free) version of this product see product # 73950 . Protocol Available protocols: Western Blotting, Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity NKG7 (E6S2A) Rabbit Monoclonal Antibody recognizes endogenous levels of total NKG7 protein. This antibody may recognize multiple predicted isoforms of NKG7 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly161 of human NKG7 protein. Background Natural killer cell granule protein 7 (NKG7, also known as GMP-17 and GIG-I) is a novel type 1 integral membrane protein expressed at high levels in lymphocytes, including natural killer (NK) cells, γδ T cells, a subset of αβ T cells, lymphokine-activated killer (LAK) cells (1), and activated T regulatory cells (2). NKG7 expression can be induced in neutrophils following GM-CSF treatment (3). NKG7 protein is localized to the membranes of cytotoxic granules. Following target cell-induced NK cell degranulation, NKG7 translocates from granules to the plasma membrane (4). Studies have also shown that NKG7 is significantly upregulated in allograft rejection patients (5-8). Alternate Names G-CSF-induced gene 1 protein; GIG-1 protein; GIG1; GMP-17; granule membrane protein 17; Granule membrane protein of 17 kDa; natural killer cell granule protein 7; natural killer cell group 7 sequence; Natural killer cell protein 7; NKG7; p15-TIA-1; Protein NKG7; Q16617 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 7-25 Source/Isotype: Rabbit IgG