CST, 85001S, ATP5B (E8A5A) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying ATP5B. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, ATP5B (E8A5A) Rabbit Monoclonal Antibody (CST #85001) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity ATP5B (E8A5A) Rabbit Monoclonal Antibody recognizes endogenous levels of total ATP5B protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu523 of human ATP5B protein. Background ATP5B (ATP5F1B) is the catalytic β subunit of the F portion of the F F ATP synthase, which is responsible for the majority of ATP synthesis in oxidative phosphorylation (1). ATP5B is present in the inner membrane of mitochondria in all types of cells, where it functions as an ATP provider and plays an important role in maintaining intracellular energy homeostasis. A high level of ATP5B is associated with cancer proliferation, metastasis, and drug resistance (2-4). An increased level of ATP5B plays an important protective role in decreasing renal fibrosis (5). Cell surface expression of ATP5B has been found in various cell types, including tumor, endothelial, and neuronal cells (6), where it is involved in multiple biological processes depending on the cell types. These include promoting viral entry (7), serving as a ligand for tumor-T cell recognition (8), generating extracellular ATP, contributing to intracellular PH homeostasis (9), and facilitating tumor metastasis (10). Inhibition of ATP5B has been proposed as a potential treatment for aggressive cancer (11,12). Alternate Names ATP synthase F1 subunit beta; ATP synthase subunit beta, mitochondrial; ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide; ATP5B; ATP5F1B; ATPB; ATPMB; ATPSB; epididymis secretory protein Li 271; HEL-S-271; MGC5231; mitochondrial ATP synthase beta subunit; mitochondrial ATP synthetase, beta subunit Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 52 Source/Isotype: Rabbit IgG