CST, 8503S, Cofilin (D3F9) Rabbit Monoclonal Antibody (HRP Conjugate)

Monoclonal Antibody for studying cofilin 1. Validated for Western Blotting. Highly specific and rigorously validated in-house, Cofilin (D3F9) Rabbit Monoclonal Antibody (HRP Conjugate) (CST #8503) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 140 mM NaCl, 3 mM KCI, 10 mM sodium phosphate (pH 7.4) dibasic, 2 mM potassium phosphate monobasic, 2 mg/mL BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Cofilin (D3F9) Rabbit Monoclonal Antibody (HRP Conjugate) detects endogenous levels of total cofilin1 protein. Species Reactivity: Human, Mouse, Rat, Monkey, Dog Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to central residues of human cofilin1 protein. Background Cofilin and actin-depolymerization factor (ADF) are members of a family of essential conserved small actin-binding proteins that play pivotal roles in cytokinesis, endocytosis, embryonic development, stress response, and tissue regeneration (1). In response to stimuli, cofilin promotes the regeneration of actin filaments by severing preexisting filaments (2). The severing activity of cofilin is inhibited by LIMK or TESK phosphorylation at Ser3 of cofilin (3-5). Phosphorylation at Ser3 also regulates cofilin translocation from the nucleus to the cytoplasm (6). Alternate Names 18 kDa phosphoprotein; CFL; CFL1; COF1; cofilin; cofilin 1; cofilin 1 (non-muscle); Cofilin-1; Cofilin, non-muscle isoform; epididymis secretory protein Li 15; HEL-S-15; p18 Specification REACTIVITY: H M R Mk Dg SENSITIVITY: Endogenous MW (kDa): 19 Source/Isotype: Rabbit IgG