CST, 8507S, KIF3A (D7G3) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying KIF3A. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, KIF3A (D7G3) Rabbit Monoclonal Antibody (CST #8507) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity KIF3A (D7G3) Rabbit Monoclonal Antibody recognizes endogenous levels of total KIF3A protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human KIF3A protein. Background Kinesin superfamily proteins (KIFs) are molecular motors that drive directional, microtubule-dependent intracellular transport of membrane-bound organelles and other macromolecules (e.g., proteins, nucleic acids). The intracellular transport functions of KIFs are fundamentally important for a variety of cellular functions, including mitotic and meiotic division, motility/migration, hormone and neurotransmitter release, and differentiation (1-4). Disruptions to KIF-mediated intracellular transport have been linked with a variety of pathologies, ranging from tumorigenesis to defects in higher order brain function such as learning and memory (4-6). Kinesin superfamily protein 3A (KIF3A) is a central component of the kinesin-2 protein complex (7). KIF3A and its paralog KIF3B bind to form a heterodimeric motor protein with ATP-dependent, plus-end-directed microtubule sliding activity (8). The tail domain of this heterodimer binds to kinesin-associated protein 3 (KAP3), which facilitates binding of the KIF3A/3B motor protein to its cargo (7,8). Recent studies in a variety of model organisms have demonstrated a critical role for kinesin-family proteins, including KIF3A, in the formation and function of cilia (9). Notably, KIF3A was shown to mediate cilia-dependent protein-protein interactions that function to transduce canonical Hedgehog signaling (10). Alternate Names FLA10; KIF3; KIF3A; kinesin family member 3A; kinesin family protein 3A; Kinesin-like protein KIF3A; KLP-20; Microtubule plus end-directed kinesin motor 3A Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 80 Source/Isotype: Rabbit IgG