CST, 85090S, COLEC11 (E9F9S) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying COLEC11. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, COLEC11 (E9F9S) Rabbit Monoclonal Antibody (CST #85090) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity COLEC11 (E9F9S) Rabbit mAb recognizes endogenous levels of total COLEC11 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu132 of human COLEC11 protein. Background Collectin-11 (COLEC11), also known as collectin kidney protein 1 (CL-K1), is a soluble pattern-recognition lectin and member of the collectin family (1). Collectins are composed of a collagen-like region and a C-terminal carbohydrate-recognition domain (CRD) (2). During embryogenesis, COLEC11 is highly expressed in craniofacial cartilage, heart, bronchi, kidney, and vertebral bodies. It may act as a guidance cue for neural crest cell migration via recognising specific carbohydrate epitopes (3). COLEC11 is secreted in the plasma and can bind a wide range of ligands, including pathogen-associated molecular patterns (PAMPs), like lipopolysaccharide (LPS) and lipoteichoic acid (LTA) (4). After binding a PAMP, COLEC11 can activate mannan-binding lectin (MBL)-associated serine proteases (MASP1, MASP2, and MASP3) to initiate complement activation and subsequent immune processes (5,6). COLEC11 has also been shown to upregulate IL-10, but downregulate IL-6 production in retinal pigment epithelial cells (RPE) and regulate antigen-presenting cell (APC) activation in a mouse model of collagen-induced rheumatoid arthritis (7,8). Elevated COLEC11 levels in the plasma are associated with the clotting disease disseminated intravascular coagulation and often lead to organ failure (9). COLEC11 has recently been identified as a tumor growth-promoting protein by directly stimulating cancer cell proliferation and by promoting a more immunosuppressive tumor microenvironment (TME). It is therefore a possible therapeutic target (10).   Alternate Names 3MC2; CL-K1; CL-K1-I; CL-K1-II; CL-K1-IIa; CL-K1-IIb; CLK1; COL11; COLEC11; Collectin K1; collectin kidney I; Collectin kidney protein 1; collectin sub-family member 11; collectin subfamily member 11; Collectin-11; DKFZp686N1868; MGC3279 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 35, 28 Source/Isotype: Rabbit IgG