CST, 85102S, CEACAM6 (E7S7Y) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CEACAM6. Validated for Western Blotting,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, CEACAM6 (E7S7Y) Rabbit Monoclonal Antibody (CST #85102) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunohistochemistry (Paraffin): 1:1600 - 1:6400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 51309 . Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin) Specificity / Sensitivity CEACAM6 (E7S7Y) Rabbit Monoclonal Antibody recognizes endogenous levels of total CEACAM6 protein. This antibody does not cross-react with CEACAM1, 5, or 8 proteins. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys246 of human CEACAM6 protein. Background Carcinoembryonic antigen (CEA)-related cell adhesion molecule 6 (CEACAM6) is a member of the CEA-related cell adhesion molecule (CEACAM) family (1). CEACAMs bind to themselves and other family members to carry out numerous cellular functions, including proliferation, signaling, differentiation, tumor suppression, and survival (2). CEACAM6 is a single-chain, glycosylphosphatidylinositol (GPI)-anchored, highly glycosylated protein that is normally expressed on epithelial cells and granulocytes (3,4). CEACAM6 mediates homophilic and heterophilic cell adhesion with other CEA-related cell adhesion molecules, such as CEACAM5 and CEACAM8 (3-6). Heterophilic interaction with CEACAM8 occurs in activated neutrophils (7), and plays a role in neutrophil adhesion to cytokine-activated endothelial cells (4). High levels of CEACAM6 expression are also observed in several different cancers (8-10). In cancer, CEACAM6 promotes tumor progression, migration, invasion, and metastasis, and therefore is considered a target for therapeutic intervention (11-13). Alternate Names carcinoembryonic antigen related cell adhesion molecule 6; Carcinoembryonic antigen-related cell adhesion molecule 6; carcinoembryonic antigen-related cell adhesion molecule 6 (non-specific cross reacting antigen); CD66c; CEA cell adhesion molecule 6; CEACAM6; CEAL; CEAM6; Cluster of Differentiation 66c; NCA; Non-specific crossreacting antigen; Normal cross-reacting antigen Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 50-200 Source/Isotype: Rabbit IgG