CST, 85114SF, CD89/FCAR (F1T9J) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying FCAR. Validated for Western Blotting,Immunohistochemistry (Paraffin). Highly specific and rigorously validated in-house, CD89/FCAR (F1T9J) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #85114) is ready to ship. Product Usage Information This product is the carrier free version of product #26013. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 26013 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity CD89/FCAR (F1T9J) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total CD89/FCAR protein. Non-specific staining was observed in pancreatic acinar cells and apical membrane of colon epithelium by immunohistochemistry. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human CD89/FCAR protein. Background Immunoglobulin alpha Fc (IgA Fc) receptor (FcαRI), also known as CD89/FCAR, is a member of the Fc receptor superfamily and is expressed as a transmembrane glycoprotein on the surface of immune cells of the myeloid lineage (1-3). Research studies have demonstrated that CD89/FCAR can bind to polymeric IgA-containing immune complexes, which mediates receptor activation, internalization, and signaling (4). Productive signaling by CD89/FCAR requires association with the FcR gamma-chain and subsequent engagement of the tyrosine kinases, Fyn and Syk, to drive activation of pro-inflammatory signaling cascades (1,5). Alternate Names CD89; CTB-61M7.2; Fc alpha receptor; Fc fragment of IgA receptor; Fc fragment of IgA, receptor for; FcalphaRI; FCAR; FCAR variant 14; IgA Fc receptor; Immunoglobulin alpha Fc receptor Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 60-80 Source/Isotype: Rabbit IgG