CST, 85124S, Nischarin (D6T4X) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying NISCH. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Nischarin (D6T4X) Rabbit Monoclonal Antibody (CST #85124) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Nischarin (D6T4X) Rabbit Monoclonal Antibody recognizes endogenous levels of total nischarin protein. This antibody detects a 60 kDa background band of unknown identity by western blot. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human nischarin protein. Background Nischarin (also known as imidazoline receptor antisera-selected protein, IRAS) was first identified through its association with the cytoplasmic domain of integrin α5, and shown to regulate cell migration and cystoskeletal organization (1). Nischarin regulates Rac-1 signaling (2), as well as the p21-activated kinase (PAK) (3) and cofilin/LIMK pathways (4). Nischarin also interacts with LKB1, regulating the migration and metastatic behavior of breast epithelial cells (5). In addition, nischarin regulates neuronal migration in rat brain (6). Research studies have implicated nischarin in the regulation of invasion and metastasis of breast cancer (7,8). Researchers have shown that nischarin is frequently downregulated in ovarian cancer, and regulates invasion through focal adhesion kinase (FAK) signaling (9). Alternate Names FLJ14425; FLJ40413; FLJ90519; hIRAS; I-1; I-1 receptor candidate protein; I1R; I1R candidate protein; Imidazoline receptor 1; imidazoline receptor antisera selected; Imidazoline receptor antisera-selected protein; imidazoline receptor candidate; Imidazoline-1 receptor; Imidazoline-1 receptor candidate protein; IR1; IRAS; KIAA0975; NISCH; Nischarin Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 200 Source/Isotype: Rabbit IgG