CST, 8513S, beta2-Adrenergic Receptor (D6H2) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying adrenergic receptor beta-2. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, beta2-Adrenergic Receptor (D6H2) Rabbit Monoclonal Antibody (CST #8513) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity beta2-Adrenergic Receptor (D6H2) Rabbit Monoclonal Antibody recognizes endogenous levels of total β2-adrenergic receptor protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human β2-adrenergic receptor protein. Background There are four major Adrenergic Receptor (AR) subtypes (α1, α2, β1, β2). Each of the subtypes has been classified by their unique responses to agonists and antagonists. Adrenergic receptors belong to the family of guanine nucleotide-binding, regulatory protein-coupled receptors (GPCR) which transverse the plasma membrane seven times. The transmembrane regions are hydrophobic and are interconnected by hydrophilic loops (1). β2-Adrenergic Receptor (β2AR) is the most studied receptor of the catecholamine system. β2AR stimulation occurs through the catecholamines epinephrine (adrenaline) and norepinephrine (noradrenaline) acting as neuromodulators in the central nervous system and as hormones in the vascular system. β2AR activation results in coupling to heterotrimeric G proteins and activation of the second messengers cAMP and phosphatidylinositol, ultimately leading to changes in cellular physiology. GPCR kinases (GRKs) terminate β2AR signaling through phosphorylation of the GPCR and by recruiting β-arrestin. β-arrestin binding uncouples the receptor from the G protein, thereby terminating G protein-mediated signaling (desensitization), and initiating clathrin-mediated endocytosis (internalization) of β2AR (2). β-adrenergic blocking agents (beta blockers) are drugs that block catecholamines from binding to βAR and are prescribed for cardiac arrhythmias, cardioprotection after myocardial infarction (heart attack), and hypertension (3). Alternate Names ADRB2; ADRB2R; ADRBR; adrenergic, beta-2-, receptor, surface; adrenoceptor beta 2; adrenoceptor beta 2 surface; B2AR; BAR; Beta-2 adrenergic receptor; Beta-2 adrenoceptor; Beta-2 adrenoreceptor; beta-2-Adrenergic Receptor; BETA2AR; catecholamine receptor Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 50-100 Source/Isotype: Rabbit IgG