CST, 8516S, Phospho-Rb (Ser807/811) (D20B12) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Rb (Ser807/Ser811) phosphate. Validated for WB,WB,WB,IP,IHC,IF,IF,F. Available in 3 sizes. Highly specific and rigorously validated in-house, Phospho-Rb (Ser807/811) (D20B12) Rabbit Monoclonal Antibody (CST #8516) is ready to ship. Product Usage Information Western Blotting: 1:1000 Fluorescent Western: 1:1000 Simple Western™: 1:10 Immunoprecipitation: 1:200 Immunohistochemistry (Paraffin): 1:200 - 1:800 Immunofluorescence (Frozen): 1:400 - 1:800 Immunofluorescence (Immunocytochemistry): 1:800 - 1:3200 Flow Cytometry (Fixed/Permeabilized): 1:200 - 1:800 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier-free (BSA and azide free) version of this product see product #www.cellsignal.com/products/primary-antibodies/phospho-rb-ser807-811-d20b12-xp-rabbit-mab-bsa-and-azide-free/41359?_=1652117273177&Ntt=41359&tahead=true">41359. Protocol Available protocols: Western Blotting, Fluorescent Western, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity Phospho-Rb (Ser807/811) (D20B12) XP Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser807/811 of human Rb protein. Background The retinoblastoma tumor suppressor protein Rb regulates cell proliferation by controlling progression through the restriction point within the G1-phase of the cell cycle (1). Rb has three functionally distinct binding domains and interacts with critical regulatory proteins including the E2F family of transcription factors, c-Abl tyrosine kinase, and proteins with a conserved LXCXE motif (2-4). Cell cycle-dependent phosphorylation by a CDK inhibits Rb target binding and allows cell cycle progression (5). Rb inactivation and subsequent cell cycle progression likely requires an initial phosphorylation by cyclin D-CDK4/6 followed by cyclin E-CDK2 phosphorylation (6). Specificity of different CDK/cyclin complexes has been observed (6-8) and cyclin D1 is required for Ser780 phosphorylation (9). Alternate Names exon 17 tumor GOS561 substitution mutation causes premature stop; GOS563 exon 17 substitution mutation causes premature stop; OSRC; p105-Rb; p110-RB1; pp110; PPP1R130; pRb; prepro-retinoblastoma-associated protein; protein phosphatase 1, regulatory subunit 130; Rb; RB transcriptional corepressor 1; RB1; retinoblastoma 1; retinoblastoma suspectibility protein; Retinoblastoma-associated protein Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 110 Source/Isotype: Rabbit IgG