CST, 8518S, Palladin (D9H2) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying palladin. Validated for Western Blotting,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, Palladin (D9H2) Rabbit Monoclonal Antibody (CST #8518) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunohistochemistry (Paraffin): 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin) Specificity / Sensitivity Palladin (D9H2) Rabbit Monoclonal Antibody recognizes endogenous levels of total palladin protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro1043 of human palladin protein. Background The actin-associated scaffold protein, palladin, is associated with multiple actin-rich structures, and plays a role in cellular migration and invasion (1). Palladin regulates the formation of invasive structures called podosomes and invadopodia (1-3). Alternative splicing results in at least three palladin isoforms, including the widely expressed 140 kDa and 90 kDa isoforms (2). Palladin has been shown to promote invasion in basal-like breast carcinoma models (4). Palladin is also upregulated in injured kidneys, and promotes migration of kidney cells to facilitate repair (5). In differentiating muscle cells, palladin regulates migration and myogenesis (6). Several research studies have focused on palladin expression and function in cancer-associated fibroblasts (CAFs). Researchers have shown that expression of palladin in stromal fibroblasts of pancreatic ductal adenocarcinoma is an indication of the effectiveness of chemotherapy (7). The cancer associated transcription factor Twist1 may require palladin and collagen alpha1 for its metastatic effect in fibroblasts (8). Alternate Names CGI-151; CGI151; FLJ22190; FLJ38193; FLJ39139; KIAA0992; MYN; myoneurin; Palladin; palladin, cytoskeletal associated protein; PALLD; PNCA1; Sarcoma antigen NY-SAR-77; SIH002 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 140, 90 Source/Isotype: Rabbit IgG