CST, 85207SF, Neurogranin (E9L8D) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying neurogranin. Validated for Western Blotting,Immunofluorescence (Frozen),ELISA+. Highly specific and rigorously validated in-house, Neurogranin (E9L8D) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #85207) is ready to ship. Product Usage Information This product is the carrier free version of product #79519. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 79519 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity Neurogranin (E9L8D) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total neurogranin protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu17 of human neurogranin protein. Background Neurogranin is a small neuronal protein that binds to calmodulin in the absence of Ca (1,2). Calmodulin binds Ca and acts as a Ca sensor to drive Ca -dependent signal transduction pathways by modulating its interaction with various kinases and phosphatases (3). In the postsynaptic compartment of the neuron, Ca  is carefully regulated and is a critical regulator of synaptic function and plasticity (4). Neurogranin is primarily expressed in the brain and is enriched in somato-dendritic compartments of projection neurons in various regions of the brain (5). Postsynaptically-enriched neurogranin likely influences Ca  or Ca /calmodulin-dependent neuronal functions, including synaptic plasticity, by binding to and releasing calmodulin in a Ca -dependent manner (6-9). Genetic variants in the gene encoding neurogranin, are linked to several neuropsychiatric diseases, including schizophrenia (10). Interestingly, increase in cerebrospinal fluid neurogranin is correlated with neurodegenerative disease progression, which suggests that neurogranin could act as a biomarker for diseases like Alzheimer's disease (11). Alternate Names calmodulin-binding protein; hng; NEUG; NEUG(55-78); Neurogranin; neurogranin (protein kinase C substrate, RC3); Ng; NRGN; protein kinase C substrate; RC3 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 15 Source/Isotype: Rabbit IgG