CST, 8523S, BAG6 Antibody

Polyclonal Antibody for studying BAG6. Validated for Western Blotting. Highly specific and rigorously validated in-house, BAG6 Antibody (CST #8523) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity BAG6 Antibody recognizes endogenous levels of total BAG6 protein. This antibody does not cross-react with other BCL2-associated athanogene (Bag) family members. Species Reactivity: Human, Mouse, Rat, Monkey, Pig Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human BAG6 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background BAG6 (BCL2-associated athanogene-6), alternately known as BAT3 (HLA-B-associated transcript 3), was originally identified as a gene within the class III region of the human major histocompatibility complex, but has subsequently been found to exhibit protein chaperone activity. BAG6, in conjunction with other chaperone proteins and ubiquitin ligases, regulates protein stability and insertion of tail-anchored membrane proteins into the endoplasmic reticulum (ER) (1-3). The BAT3 complex, consisting of BAG6, TRC35, and Ubl4a localizes to ribosomes synthesizing membrane proteins and facilitates tailed-anchored protein capture by TRC40 and subsequent insertion of the nascent protein in to the ER membrane (4,5). BAG6 also plays a critical role in clearing cells of mis-folded and mis-localized peptides via ER-associated degradation and the ubiquitin-proteasome system (1,6,7). BAG6 may also act as a chaperone for glycoproteins through its interaction with DERLIN2 (8). In addition to its role as a chaperone, BAG6 has also been implicated in regulating chromatin structure and gene expression. For example, BAG6 and SET1A act as binding partners for BORIS to effect changes of chromatin structure and gene expression (9). Similarly, increased expression of BAG6 induces p300-mediated acetylation of p53, which is required for DNA damage response (10). BAG6 has also been found to interact with TGF-β, and in so doing acts as a positive regulator of TGF-β1 stimulation of type 1 collagen expression (11). BAG6 also suppresses bone morphogenic protein (BMP) signaling via its interaction with and regulation of small C-terminal domain phosphatase (SCP) that dephosphorylates SMAD proteins, resulting in subsequent termination of BMP-mediated events (12). Alternate Names BAG cochaperone 6; BAG family molecular chaperone regulator 6; BAG-6; BAG6; BAT3; BCL2 associated athanogene 6; BCL2-associated athanogene 6; D6S52E; G3; HLA-B associated transcript 3; HLA-B associated transcript-3; HLA-B-associated transcript 3; Large proline-rich protein BAG6; Large proline-rich protein BAT3; Protein G3; Protein Scythe; scythe Specification REACTIVITY: H M R Mk Pg SENSITIVITY: Endogenous MW (kDa): 150 SOURCE: Rabbit