CST, 85303T, IP3R3/ITPR3 (F3L2A) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying IP3R3. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, IP3R3/ITPR3 (F3L2A) Rabbit Monoclonal Antibody (CST #85303) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity IP3R3/ITPR3 (F3L2A) Rabbit Monoclonal Antibody recognizes endogenous levels of total IP3R3/ITPR3 protein. This antibody detects a 120 kDa protein of unknown identity in some cell lines. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human IP3R3/ITPR3 protein. Background Inositol 1,4,5-trisphosphate receptors (IP3Rs) are multi-transmembrane Ca2+ release channels located predominantly in the endoplasmic reticulum (ER) of all cell types (1,2). There are three distinct isoforms of IP3Rs in mammalian cells, IP3R1, IP3R2, and IP3R3, encoded by genes , , and , respectively (3). Each isoform exhibits a distinct expression pattern. IP3R1 is highly expressed in neurons, IP3R2 in cardiomyocytes and hepatocytes, and IP3R3 in rapidly proliferating cultured cells (4). IP3R3 is activated mainly by IP3-binding due to increased intracellular IP3 levels. Its Ca2+ releasing function is also regulated by other mechanisms, including intracellular Ca2+ levels, ATP levels, Akt-dependent C-terminal phosphorylation, interaction with Bcl-2 or G protein-coupled receptor kinase-interacting proteins 1 and 2 (5). IP3R3 plays important roles in various diseases, including cancer and neurological disorders (6). Depending on the cancer type and cellular context, the protein can act as an oncogene or tumor suppressor (7-9). By controlling Ca2+ release, the protein promotes insulin secretion in pancreatic beta cells and fluid secretion in salivary gland acini (10-12). Alternate Names FLJ36205; inositol 1,4,5-triphosphate receptor, type 3; Inositol 1,4,5-trisphosphate receptor type 3; Inositol 1,4,5-trisphosphate receptor type 3 (Type 3 inositol 1,4,5-trisphosphate receptor) (Type 3 InsP3 receptor) (IP3 receptor isoform 3) (InsP3R3); InsP3R3; IP3 receptor; IP3 receptor isoform 3; IP3R; IP3R 3; IP3R3; ITPR3; Type 3 inositol 1,4,5-trisphosphate receptor; Type 3 InsP3 receptor Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 320 Source/Isotype: Rabbit IgG