CST, 85313S, CD69 (FN50) Mouse Monoclonal Antibody (PE-Cy7® Conjugate)

Monoclonal Antibody for studying CD69. Validated for Flow Cytometry (Live). Highly specific and rigorously validated in-house, CD69 (FN50) Mouse Monoclonal Antibody (PE-Cy7^® Conjugate) (CST #85313) is ready to ship. Product Usage Information Flow Cytometry (Live): 1:20 Storage Supplied in 10 mM NaH 2 PO 4 , 150 mM NaCl, 0.09% NaN 3 , 0.1% gelatin, pH 7.2. This product is stable for 6 months when stored at 4ºC. Do not aliquot the antibody. Protect from light. Do not freeze. Protocol Available protocols: Flow Cytometry (Live) Specificity / Sensitivity CD69 (FN50) Mouse Monoclonal Antibody (PE-Cy7 Species Reactivity: Human Source / Purification This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation. Background CD69, also known as Leu-23, is a type II transmembrane glycoprotein that is expressed on the surface of T cells, B cells, and NK cells (1,2). This phosphorylated disulfide-linked 28 to 32-kDa homodimer is constitutively expressed on a subset of thymocytes and platelets. It also acts as an activation antigen of lymphocytes, NK cells, neutrophils, and eosinophils (1-6). Studies have shown that stimulation of the T cell receptor (TCR) increases the expression of CD69 on the cell surface. The ability to detect the level of CD69 expression after TCR activation makes CD69 an ideal indicator of T cell activation (1). The FN50 antibody is widely used as a marker for T cell activation (7). Alternate Names Activation inducer molecule; activation inducer molecule (AIM/CD69); AIM; BL-AC/P26; C-type lectin domain family 2 member C; C-type lectin domain family 2, member C; CD69; CD69 antigen (p60, early T-cell activation antigen); CD69 molecule; CLEC2C; EA1; Early activation antigen CD69; early lymphocyte activation antigen; Early T-cell activation antigen p60; GP32/28; Leukocyte surface antigen Leu-23; MLR-3 Specification REACTIVITY: H SENSITIVITY: Endogenous Source/Isotype: Mouse IgG1 kappa