CST, 85315SF, Caspase-1 (D7F10) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying Casp1. Validated for Western Blotting. Highly specific and rigorously validated in-house, Caspase-1 (D7F10) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #85315) is ready to ship. Product Usage Information This product is the carrier free version of product #3866. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 3866 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity Caspase-1 (D7F10) Rabbit Monoclonal Antibody (BSA and Azide Free) detects endogenous levels of full length human Caspase-1. The activated p20 subunit was detected by over-expression. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues within the p20 subunit of human caspase-1. Background Caspase-1, or interleukin-1ß converting enzyme (ICE/ICEα), is a class I cysteine protease, which also includes caspases -4, -5, -11, and -12. Caspase-1 cleaves inflammatory cytokines such as pro-IL-1ß and interferon-γ inducing factor (IL-18) into their mature forms (1,2). Like other caspases, caspase-1 is proteolytically activated from a proenzyme to produce a tetramer of its two active subunits, p20 and p10. Caspase-1 has a large amino-terminal pro-domain that contains a caspase recruitment domain (CARD). Overexpression of caspase-1 can induce apoptosis (3). Mice deficient in caspase-1, however, have no overt defects in apoptosis but do have defects in the maturation of pro-IL-1β and are resistant to endotoxic shock (4,5). At least six caspase-1 isoforms have been identified, including caspase-1 α, β, γ, δ, ε, and ζ (6). Most caspase-1 isoforms (α, β, γ, and δ) produce products between 30-48 kDa and induce apoptosis upon overexpression. Caspase-1 ε typically contains only the p10 subunit, does not induce apoptosis, and may act as a dominant negative. The widely expressed ζ isoform of caspase-1 induces apoptosis and lacks 39 amino-terminal residues found in the α isoform (6). Activation of caspase-1 occurs through an oligomerization molecular platform designated the "inflammasome" that includes caspase-5, Pycard/Asc, and NALP1 (7). Alternate Names CASP-1; CASP1; CASP1 nirs variant 1; Caspase 1; caspase 1, apoptosis-related cysteine peptidase; caspase 1, apoptosis-related cysteine peptidase (interleukin 1, beta, convertase); caspase 1, apoptosis-related cysteine protease (interleukin 1, beta, convertase); Caspase-1; Caspase-1 subunit p10; Caspase-1 subunit p20; caspase1; Caspase1; ICE; IL-1 beta-converting enzyme; IL-1BC; IL1B-convertase; IL1BC; IL1BCE; interleukin 1-B converting enzyme; interleukin 1-beta convertase; interleukin 1, beta, convertase; Interleukin-1 beta convertase; Interleukin-1 beta-converting enzyme; p45 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 48, 20 Source/Isotype: Rabbit IgG