CST, 85325S, PICK1 (D6W7M) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying PICK1. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, PICK1 (D6W7M) Rabbit Monoclonal Antibody (CST #85325) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity PICK1 (D6W7M) Rabbit Monoclonal Antibody recognizes endogenous levels of total PICK1 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro20 of human PICK1 protein. Background PICK1, or Protein interacting with C-kinase 1, is a cytosolic adaptor protein composed of an N-terminus PDZ domain and a C-terminus BAR domain that allow protein and membrane interactions, respectively (1,2). PICK1 regulates endosomal trafficking and surface expression of AMPA receptors and is therefore involved in synaptic plasticity (3). PICK1 is a negative regulator of Arp2/3-dependent actin polymerization and also for the development of neuronal architecture (4). Finally, increasing evidence indicates that PICK1 expression is upregulated in a number cancers (5), and that PICK1 interacts with proteins involved in the promotion of tumorigenesis such as Ephrin receptors, Coxsackie-adenovirus receptor (CAR), and ErbB2/Her2 (6,7). Alternate Names MGC15204; PICK; PICK1; PRKCA-binding protein; PRKCABP; Protein interacting with C kinase 1; protein interacting with PRKCA 1; Protein kinase C-alpha-binding protein; protein kinase C, alpha binding protein Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 50 Source/Isotype: Rabbit IgG