CST, 8532S, FYVE-CENT Antibody

Polyclonal Antibody for studying FYVE-CENT. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, FYVE-CENT Antibody (CST #8532) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity FYVE-CENT Antibody recognizes endogenous levels of total FYVE-CENT protein. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu595 of human FYVE-CENT protein. Antibodies are purified by protein A and peptide affinity chromatography. Background FYVE-CENT (ZFYVE26) contains an FYVE zinc finger domain characterized by conserved R(R/RK) and HHCRxCG motifs (1). Phosphatidylinositol 3 phosphate binds to FYVE-CENT via the FYVE domain and this binding is crucial in phosphoinositide (PtdIns3P)-regulated cytokinesis (1-3). Research evidence suggests that during cytokinesis, FYVE-CENT directly interacts with TTC19 and is recruited to the midbody by the kinesin protein KIF13A (4). FYVE-CENT may also be involved in the tumor suppressor function of Beclin-1 (5). Researchers have linked the defects in FYVE-CENT to hereditary spastic paraplegia, a condition characterized by deterioration of the corpus callosum of the brain (6). Alternate Names DKFZp686F19106; DKFZp781H1112; FYVE domain-containing centrosomal protein; FYVE-CENT; KIAA0321; Spastizin; SPG15; ZFY26; ZFYVE26; Zinc finger FYVE domain-containing protein 26; zinc finger FYVE-type containing 26; zinc finger, FYVE domain containing 26 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 285 SOURCE: Rabbit