CST, 85393SF, p22phox (E7Y1F) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying CYBA. Validated for WB,IHC,IF,IF. Highly specific and rigorously validated in-house, p22phox (E7Y1F) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #85393) is ready to ship. Product Usage Information This product is the carrier free version of product #37570. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 37570 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity p22phox (E7Y1F) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total p22phox protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro187 of human p22phox protein. Background The p22phox protein is a critical component of the membrane-bound NADPH Oxidase of phagocytes that generate superoxide anion and alter the redox states in cells. p22phox associates with the catalytic subunit gp91phox (NOX2), NOX1, NOX3, or NOX4 to form the active NADPH Oxidase. The enzymatic complex includes cytosolic subunits p40phox, p47phox, p67phox, and Rac2 that are recruited to the membrane-bound subunits, subsequently generating superoxide anion as the final product, serving as a microbial killing system (1,2). Multiple phosphorylation sites in each subunit of these oxidases potentiate its activation, including the phosphorylation of p22phox at Thr147 epitope facing the cytoplasmic space (3-5). NADPH Oxidase is associated with several diseases, including hyperglycemia, cancer, inflammation and vascular cases, as well as neurodegenerative diseases (6), including its activation by SOD1 mutants in Amyotrophic Lateral Sclerosis (7), and in Alzheimer's disease where NADPH Oxidase activation exacerbates the effects of fibrillary tangles and β-amyloid plaques, causing neuronal death (8,9). Alternate Names CGD4; CY24A; CYBA; cytochrome b light chain; cytochrome b-245 alpha chain; Cytochrome b-245 light chain; cytochrome b-245, alpha polypeptide; cytochrome b, alpha polypeptide; Cytochrome b(558) alpha chain; cytochrome b(558) alpha-subunit; Cytochrome b558 subunit alpha; flavocytochrome b-558 alpha polypeptide; Neutrophil cytochrome b 22 kDa polypeptide; p22 phagocyte B-cytochrome; p22-phox; p22phox; Superoxide-generating NADPH oxidase light chain subunit Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 22 Source/Isotype: Rabbit IgG