CST, 85449S, JMJD7 (E8J8Q) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying JMJD7. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, JMJD7 (E8J8Q) Rabbit Monoclonal Antibody (CST #85449) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity JMJD7 (E8J8Q) Rabbit Monoclonal Antibody recognizes endogenous levels of total JMJD7 protein. Species Reactivity: Human, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro251 of human JMJD7 protein. Background The methylation state of lysine residues in histone proteins is a major determinant of the formation of active and inactive regions of the genome and is crucial for proper programming of the genome during development (1,2). Jumonji C (JmjC) domain-containing proteins represent the largest class of potential histone demethylase proteins (3). The JmjC domain can catalyze the demethylation of mono-, di-, and tri-methyl lysine residues via an oxidative reaction that requires iron and α-ketoglutarate (3). Based on homology, both humans and mice contain at least 30 such proteins, which can be divided into 7 separate families (3). JMJD7 is a member of the less studied JmjC family of oxygenates that catalyze formation of alcohol products. JMJD7 uniquely catalyzes (3S)-lysyl hydroxylation in members of the Translation Factor GTPase family (4). JMJD7, along with JMJD5, have also been found to function as proteases capable of cleaving histone tails containing methylated arginine (5,6). The gene can fuse with neighboring gene to drive head and neck squamous cell carcinomas (7). Alternate Names Bifunctional peptidase and (3S)-lysyl hydroxylase JMJD7; JmjC domain-containing protein 7; JMJD7; jumonji domain containing 7; Jumonji domain-containing protein 7; L-lysine (3S)-hydroxylase JMJD7 Specification REACTIVITY: H R Mk SENSITIVITY: Endogenous MW (kDa): 35, 40 Source/Isotype: Rabbit IgG