CST, 85460T, MYST3 (E3P5T) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying MYST3. Validated for Western Blotting,Immunoprecipitation,Chromatin IP. Available in 2 sizes. Highly specific and rigorously validated in-house, MYST3 (E3P5T) Rabbit Monoclonal Antibody (CST #85460) is ready to ship. Product Usage Information For optimal ChIP results, use 10 μL of antibody and 10 μg of chromatin (approximately 4 × 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits. Western Blotting: 1:1000 Immunoprecipitation: 1:50 Chromatin IP: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Chromatin IP Specificity / Sensitivity MYST3 (E3P5T) Rabbit Monoclonal Antibody recognizes endogenous levels of total MYST3 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro927 of human MYST3 protein. Background MYST3, also known as monocytic leukemia zinc finger protein (MOZ) and lysine acetyltransferase 6A (KAT6A), is a member of the MYST (MOZ, YBF2, SAS2, and TIP60) family of histone acetyltransferases (1,2). First discovered as a fusion partner of CREBBP in acute myeloid leukemia, MYST3 contributes to gene expression and segment identity during development (3-6). MYST3 forms an evolutionarily conserved complex with ING5, EAF6, and BRD1 and has been shown to be a coactivator for many different transcription factors, including PU.1, NRF2, and Runx family members (7-9). MYST3 is critical in hematopoietic stem cell maintenance, where it acts synergistically with polycomb member BMI1 (10). Inhibitors of MYST3 are being investigated for therapeutic value as they can induce senescence and decrease tumor growth (11). Alternate Names ARTHS; Histone acetyltransferase KAT6A; Histone acetyltransferase MYST3; K(lysine) acetyltransferase 6A; KAT6A; lysine acetyltransferase 6A; MGC167033; Monocytic leukemia zinc finger protein; MOZ; MOZ, YBF2/SAS3, SAS2 and TIP60 protein 3; MRD32; MYST histone acetyltransferase (monocytic leukemia) 3; MYST-3; MYST3; runt-related transcription factor binding protein 2; Runt-related transcription factor-binding protein 2; RUNXBP2; ZC2HC6A; Zinc finger protein 220; ZNF220 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 320 Source/Isotype: Rabbit IgG