CST, 85470SF, HOXB13 (D7N8O) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying HOXB13. Validated for Western Blotting,Immunohistochemistry (Paraffin). Highly specific and rigorously validated in-house, HOXB13 (D7N8O) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #85470) is ready to ship. Product Usage Information This product is the carrier free version of product #90944. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 90944 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity HOXB13 (D7N8O) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total HOXB13 protein. Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly11 of human HOXB13 protein. Background HOXB13 is a member of the HOXB cluster which, along with the HOXA, HOXC, and HOXD clusters, governs embryonic patterning along the cranio-caudal axis (1,2). HOXB13 plays a key role in the development of the ventral prostate, where it is expressed highly from the embryonic stage through adulthood (3,4). Research studies have shown that both overexpression and RNA interference can inhibit the growth of prostate cancer cells. HOXB13 can function as a tumor suppressor by negatively regulating growth through repression of TCF4 and androgen receptor (AR) signaling (4,5). However, HOXB13 has also been shown to be overexpressed in more invasive prostate cancers, breast and ovarian cancers, and hepatocellular carcinomas (6-9). A common germline mutation G84E in the HOXB13 protein has recently been found to be associated with significant increased risk of prostate cancer (10). Currently, HOXB13 is being evaluated as a marker for metastatic lesions of prostate origin (11,12). Alternate Names homeo box B13; homeobox B13; Homeobox protein Hox-B13; HOXB13; HPC9; HXB13; PSGD Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 30 Source/Isotype: Rabbit IgG