CST, 85570S, MLX (D8G6W) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying MLX. Validated for Western Blotting,Immunoprecipitation,Chromatin IP. Available in 2 sizes. Highly specific and rigorously validated in-house, MLX (D8G6W) Rabbit Monoclonal Antibody (CST #85570) is ready to ship. Product Usage Information For optimal ChIP results, use 5 μl of antibody and 10 μg of chromatin (approximately 4 x 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits. Western Blotting: 1:1000 Immunoprecipitation: 1:100 Chromatin IP: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Chromatin IP Specificity / Sensitivity MLX (D8G6W) Rabbit Monoclonal Antibody recognizes endogenous levels of total MLX protein. This antibody detects all known isoforms of MLX, including MLX alpha, beta, gamma, delta, and epsilon. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val286 of human MLX protein. Background Max-like protein X (MLX), also known as transcription factor-like protein 4 (TCFL4), is a member of the Myc/Max/Mad network of transcriptional regulator proteins that share a common basic-helix-loop-helix-leucine zipper (bHLH-Zip) motif required for dimerization and DNA-binding (1,2). MLX is ubiquitously expressed in most cell lines and functions as a binding partner for MLXIP (also known as MondoA) and MLXIPL (also known as ChREBP) (1,2). MLX/MLXIP and MLX/MLXIPL heterodimers function to regulate glucose homeostasis by sensing glucose metabolites in the cell. These heterodimeric protein complexes reside mainly in the cytoplasm and mitochondria of cells grown in low glucose, and translocate to the nucleus upon increased intracellular glucose levels to activate transcription of downstream target genes (1,2). MLX/MLXIP is required for the deregulated Myc-induced reprogramming of multiple metabolic pathways during oncogenesis (3). Alternate Names BHLHD13; BigMax protein; Class D basic helix-loop-helix protein 13; MAD7; MAX dimerization protein MLX; Max-like bHLHZip protein; Max-like protein X; MLX; MLX, MAX dimerization protein; MXD7; Protein BigMax; TCFL4; TF4; transcription factor-like 4; Transcription factor-like protein 4 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 30 to 50 Source/Isotype: Rabbit IgG