CST, 8559T, TPX2 Antibody

Polyclonal Antibody for studying TPX2. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, TPX2 Antibody (CST #8559) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity TPX2 Antibody recognizes endogenous levels of total TPX2 protein. Species Reactivity: Human, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys420 of human TPX2 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background The Ras family small GTPase Ran is involved in nuclear envelope formation, assembly of the mitotic spindle, and nuclear transport (1,2). TPX2, a target of active Ran (RanGTP), is a microtubule nucleating protein. TPX2 is inactive when bound to nuclear importin-alpha. RanGTP activity disrupts this interaction, relieving inhibition of TPX2 (3). TPX2 binding activates Aurora A kinase and promotes its localization to the mitotic spindle (4,5). DNA damage in mitosis leads to loss of interaction between Aurora A and TPX2 and inactivation of Aurora A kinase (6). TPX2 is highly expressed in pancreatic cancer cells, and knockdown of TPX2 expression in these cells is associated with increased sensitivity to paclitaxel (7). Alternate Names C20orf1; C20orf2; Differentially expressed in cancerous and non-cancerous lung cells 2; differentially expressed in lung cells; DIL-2; DIL2; FLS353; GD:C20orf1; HCA519; HCTP4; Hepatocellular carcinoma-associated antigen 519; Hepatocellular carcinoma-associated antigen 90; p100; preferentially expressed in colorectal cancer; Protein fls353; REPP86; restricted expression proliferation associated protein 100; Restricted expression proliferation-associated protein 100; Targeting protein for Xklp2; TPX2; TPX2 microtubule nucleation factor; TPX2, microtubule-associated protein homolog; TPX2, microtubule-associated, homolog; TPX2, microtubule-associated, homolog (Xenopus laevis) Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 100 SOURCE: Rabbit