CST, 85624T, Peripherin (F3J1R) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying peripherin mouse. Validated for Western Blotting,Immunofluorescence (Frozen),Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, Peripherin (F3J1R) Rabbit Monoclonal Antibody (CST #85624) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunofluorescence (Frozen): 1:50 - 1:200 Immunofluorescence (Immunocytochemistry): 1:50 - 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity Peripherin (F3J1R) Rabbit Monoclonal Antibody recognizes endogenous levels of total peripherin protein. This antibody detects a peripherin dimer at 140 kDa and may also detect other peripherin isoforms. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of mouse peripherin protein. Background The cytoskeleton consists of three types of cytosolic fibers: actin microfilaments, intermediate filaments, and microtubules. Peripherin is a class-III intermediate filament protein selectively expressed in peripheral nervous system (PNS) neurons (1). Several peripherin isoforms exist through alternative splicing, with the dominant isoform occurring at 58 kDa and minor isoforms occurring at 48, 45, 32, and 25 kDa (2,3). Additionally, an aggregate-forming 28 kDa splice isoform of peripherin has been shown to be upregulated in Amyotrophic Lateral Sclerosis, suggesting a role in disease pathology (3). Functionally, peripherin plays an important role in neurite outgrowth, axonal transport, and myelination; it also interacts with proteins responsible for vesicular trafficking (4). Because of its nearly exclusive expression in PNS neurons, it may be a promising blood biomarker of PNS axonal damage (5). Alternate Names PERI; Peripherin; peripherin 1; Prph; Prph1 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 58 Source/Isotype: Rabbit IgG