CST, 85652T, Musashi (D46A8) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Musashi-1. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Musashi (D46A8) Rabbit Monoclonal Antibody (CST #85652) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Musashi (D46A8) Rabbit Monoclonal Antibody recognizes endogenous levels of total Musashi-1 and Musashi-2 proteins. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala100 of human Musashi-1 protein. Background Musashi-1 and Musashi-2 are RNA-binding proteins which play a role in asymmetric cell division of ectodermal precursor cells by regulating the translation of target mRNA. Both family members augment Notch signaling and repress the translation of m-Numb, a protein that positively modulates differentiation of neural stem cells into neurons. Thus, Musashi contributes to the maintenance of neural stem cells (1). While Musashi-1 is frequently used as a marker for proliferating neural precursor cells, it is also expressed in epithelial stem cells including intestinal and mammary gland stem cells (2-4). Alternate Names MSI1; MSI1H; musashi 1; musashi homolog 1 (Drosophila); musashi RNA binding protein 1; Musashi-1; musashi1; RNA-binding protein Musashi homolog 1 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 35 Source/Isotype: Rabbit IgG