CST, 85724S, Cadherin-17 (E5J8Z) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CDH17. Validated for Western Blotting,Immunohistochemistry (Paraffin),Flow Cytometry (Fixed/Permeabilized). Available in 2 sizes. Highly specific and rigorously validated in-house, Cadherin-17 (E5J8Z) Rabbit Monoclonal Antibody (CST #85724) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunohistochemistry (Paraffin): 1:250 - 1:1000 Flow Cytometry (Fixed/Permeabilized): 1:50 - 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier-free (BSA and azide free) version of this product see product # 53536 . Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity Cadherin-17 (E5J8Z) Rabbit Monoclonal Antibody recognizes endogenous levels of total cadherin-17 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human cadherin-17 protein. Background Cadherin-17/Liver-Intestine-cadherin (CDH17/LI-cadherin) is a type-I transmembrane glycoprotein that belongs to the 7D-cadherin superfamily. Unlike classical cadherins, CDH17 is characterized by an extracellular domain with seven cadherin repeats and a short intracellular domain (1). CDH17 is a calcium-dependent homotypic cell-cell adhesion molecule that, under normal physiologic conditions in humans, is selectively expressed on the basolateral surface of epithelia lining the small intestine and colon (2). Research studies have demonstrated that CDH17 is aberrantly overexpressed in human gastric cancer and may serve as a novel oncogenic biomarker for this disease (3,4). At the molecular level, research studies have suggested that CDH17 exerts its oncogenicity in the context of gastric cancer through its ability to engage both NF-κB and MAPK signaling cascades (5,6). Alternate Names CAD17; cadherin 17; cadherin 17, LI cadherin (liver-intestine); cadherin-16; Cadherin-17; CDH16; CDH17; FLJ26931; HPT-1; HPT-1 cadherin; HPT1; human intestinal peptide-associated transporter HPT-1; human peptide transporter 1; Intestinal peptide-associated transporter HPT-1; LI cadherin; LI-cadherin; Liver-intestine cadherin; MGC138218; MGC142024 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 120 Source/Isotype: Rabbit IgG