CST, 8574T, MYPT1 (D6C1) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying MYPT1. Validated for WB,IP,IHC,IF. Available in 2 sizes. Highly specific and rigorously validated in-house, MYPT1 (D6C1) Rabbit Monoclonal Antibody (CST #8574) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:200 Immunohistochemistry (Paraffin): 1:50 - 1:200 Immunofluorescence (Immunocytochemistry): 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 24817 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity MYPT1 (D6C1) Rabbit Monoclonal Antibody recognizes endogenous levels of total MYPT1 protein. Species Reactivity: Human, Monkey, Dog Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human MYPT1 protein. Background Protein phosphatase 1 (PP1) is a ubiquitous eukaryotic protein serine/threonine phosphatase involved in the regulation of various cell functions. Substrate specificity is determined by the binding of a regulatory subunit to the PP1 catalytic subunit (PP1c). It is estimated that over fifty different regulatory subunits exist (1). The myosin phosphatase holoenzyme is composed of three subunits: PP1c, a targeting/regulatory subunit (MYPT/myosin-binding subunit of myosin phosphatase), and a 20 kDa subunit of unknown function (M20). MYPT binding to PP1cδ alters the conformation of the catalytic cleft and increases enzyme activity and specificity (2). Two MYPT isoforms that are 61% identical have been described. MYPT1 is widely expressed, while MYPT2 expression appears to be exclusive to heart and brain (3). Related family members include MBS85, MYPT3, and TIMAP (4). Myosin phosphatase regulates the interaction of actin and myosin in response to signaling through the small GTPase Rho. Rho activity inhibits myosin phosphatase via Rho-associated kinase (ROCK). Phosphorylation of MYPT1 at Thr696 and Thr853 results in phosphatase inhibition and cytoskeletal reorganization (5,6). Alternate Names GUBS; M130; MBS; MGC133042; myosin binding subunit; Myosin phosphatase target subunit 1; Myosin phosphatase-targeting subunit 1; myosin phosphatase, target subunit 1; MYPT1; PPP1R12A; Protein phosphatase 1 regulatory subunit 12A; protein phosphatase 1, regulatory (inhibitor) subunit 12A; Protein phosphatase myosin-binding subunit Specification REACTIVITY: H Mk Dg SENSITIVITY: Endogenous MW (kDa): 140 Source/Isotype: Rabbit IgG