CST, 85758S, CD162/PSGL-1 (F8D5X) Rabbit Monoclonal Antibody (Alexa Fluor® 647 Conjugate)

Monoclonal Antibody for studying SELPLG. Validated for Flow Cytometry (Live). Highly specific and rigorously validated in-house, CD162/PSGL-1 (F8D5X) Rabbit Monoclonal Antibody (Alexa Fluor^® 647 Conjugate) (CST #85758) is ready to ship. Product Usage Information Flow Cytometry (Live): 1:50 Storage Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze. Protocol Available protocols: Flow Cytometry (Live) Specificity / Sensitivity CD162/PSGL-1 (F8D5X) Rabbit Monoclonal Antibody (Alexa Fluor Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly281 of human CD162/PSGL-1 protein. Background P-selectin glycoprotein ligand-1 (PSGL-1/CD162) is a disulfide-linked homodimer expressed on the cell surface of lymphoid and myeloid cells as a type I transmembrane sialoglycoprotein (1,2). The mature protein backbone of the extracellular domain of PSGL-1 is extensively modified with N-linked glycans, O-linked glycans, sulfated tyrosine residues, and sialyl-Lewis X epitopes (3). Collectively, these modifications modulate high affinity interactions between PSGL-1 and multiple selectins, which facilitates immune cell trafficking to primary and secondary lymphoid organs (4-8). Alternate Names CD162; CLA; cutaneous lymphocyte-associated associated antigen; P-selectin glycoprotein ligand 1; PSGL-1; PSGL1; Selectin P ligand; SELPL; SELPLG Specification REACTIVITY: H SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG