CST, 8576S, GOPC (D10A12) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying FIG. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, GOPC (D10A12) Rabbit Monoclonal Antibody (CST #8576) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity GOPC (D10A12) Rabbit Monoclonal Antibody detects endogeneous levels of total GOPC protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Phe31 of human GOPC protein. Background GOPC (FIG) was originally identified as a Golgi-associated, PDZ domain containing protein. It has two coiled-coil domains (CC1 and CC2) located in the amino-terminal region and a PDZ domain in the carboxy-terminal region (1). The CC2 domain and its adjacent linker region mediate the association of GOPC with the golgi protein golgin-160 and the Q-SNARE protein syntaxin 6 (1,2). The PDZ domain of GOPC interacts with the carboxy terminus of target proteins to mediate target protein vesicular trafficking and surface expression (3-6). Fusion of the corresponding GOPC gene with the ROS tyrosine kinase oncogene has been detected in some glioblastomas. The resulting GOPC-ROS fusion protein is targeted to the golgi apparatus where a constitutively activate ROS tyrosine kinase can mediate tumor formation (7,8). Alternate Names CAL; CFTR-associated ligand; dJ94G16.2; dJ94G16.2 PIST; FIG; Fused in glioblastoma; golgi associated PDZ and coiled-coil motif containing; Golgi associated PDZ and coiled-coil motif containing protein; golgi-associated PDZ and coiled-coil motif containing; golgi-associated PDZ and coiled-coil motif containing protein; Golgi-associated PDZ and coiled-coil motif-containing protein; GOPC; GOPC1; PDZ protein interacting specifically with TC10; PDZ/coiled-coil domain binding partner for the rho-family GTPase TC10; PIST Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 59 Source/Isotype: Rabbit IgG