CST, 8577T, PRP4K (D27A1) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying PRP4. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, PRP4K (D27A1) Rabbit Monoclonal Antibody (CST #8577) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity PRP4K (D27A1) Rabbit Monoclonal Antibody recognizes endogenous levels of total PRP4K protein. Species Reactivity: Human, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys659 of human PRP4K protein. Background PRP4K (PRP4 kinase) belongs to the Clk (Cdc-like kinase) family that recognizes serine/arginine-rich substrates (1). PRP4K is involved in pre-mRNA splicing and transcriptional regulation by interacting with components of both the U5 snRNP and the NCoR Deacetylase complexes (2,3). PRP6 and PRP31 have been shown to be direct substrates of PRP4K; their phosphorylation by PRP4K contributes to the assembly of a stable spliceosomal B complex (4). Alternate Names dJ1013A10.1; dJ1013A10.1 (PRP4 protein kinase homolog); KIAA0536; PR4H; pre-mRNA processing factor 4B; protein-serine/threonine kinase; PRP4; PRP4 kinase; PRP4 pre-mRNA processing factor 4 homolog B; PRP4 pre-mRNA processing factor 4 homolog B (yeast); PRP4 pre-mRNA-processing factor 4 homolog; PRP4B; PRP4H; PRP4K; PRPF4B; Serine/threonine-protein kinase PRP4 homolog; serine/threonine-protein kinase PRP4K Specification REACTIVITY: H R Mk SENSITIVITY: Endogenous MW (kDa): 150 Source/Isotype: Rabbit IgG