CST, 85786S, Decorin (E2N2C) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Decorin. Validated for Western Blotting,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, Decorin (E2N2C) Rabbit Monoclonal Antibody (CST #85786) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunohistochemistry (Paraffin): 1:100 - 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 55868 . Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin) Specificity / Sensitivity Decorin (E2N2C) Rabbit Monoclonal Antibody recognizes endogenous levels of total decorin protein. This protein is heavily glycosylated, therefore, multiple bands or a smear may result from western blot analysis using this antibody. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro216 of human decorin protein. Background Decorin, named for its role in "decorating" type I collagen fibrils, is a collagen-binding proteoglycan that belongs to the small leucine-rich proteoglycan (SLRP) family (1). Through its binding to type I collagens and other important matrix proteins (e.g., fibronectin, thrombospondin, TGF-β), decorin plays a critically important role in assembly, maintenance, and physiological functions of the extracellular matrix. Notably, multiple research studies have reported anti-tumor functions for decorin and the related protein biglycan (2-4), implicating SLRP proteins as functionally important components of the tumor microenvironment (5). The anti-tumor effects of decorin have been ascribed to its interactions with cell surface receptors and associated signaling pathways (6), including those mediating inflammation, autophagy, and cell proliferation (7-9). Alternate Names Bone proteoglycan II; CSCD; DCN; Decorin; decorin proteoglycan; dermatan sulphate proteoglycans II; DSPG2; PG-S2; PG40; PGII; PGS2; proteoglycan core protein; SLRR1B; small leucine-rich protein 1B Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 40, 100 Source/Isotype: Rabbit IgG