CST, 8578T, Acetyl-CoA Carboxylase 2 (D5B9) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying acetyl-CoA carboxylase 2. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Acetyl-CoA Carboxylase 2 (D5B9) Rabbit Monoclonal Antibody (CST #8578) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Acetyl-CoA Carboxylase 2 (D5B9) Rabbit Monoclonal Antibody recognizes endogenous levels of total acetyl-CoA carboxylase 2 protein and does not cross-react acetyl-CoA carboxylase 1. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val1400 of human acetyl-CoA carboxylase 2 protein. Background Acetyl-CoA carboxylase (ACC) catalyzes the carboxylation of acetyl-CoA to malonyl-CoA (1). It is the key enzyme in the biosynthesis and oxidation of fatty acids (1). In rodents, the 265 kDa ACC1 (ACCα) form is primarily expressed in lipogenic tissues, while 280 kDa ACC2 (ACCβ) is the main isoform in oxidative tissues (1,2). However, in humans, ACC2 is the predominant isoform in both lipogenic and oxidative tissues (1,2). Phosphorylation by AMPK at Ser79 or by PKA at Ser1200 inhibits the enzymatic activity of ACC (3). ACC is a potential target of anti-obesity drugs (4,5). Alternate Names ACACB; ACC-beta; ACC2; ACCB; Acetyl-CoA carboxylase 2; acetyl-CoA carboxylase beta; acetyl-Coenzyme A carboxylase beta; Biotin carboxylase; HACC275 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 280 Source/Isotype: Rabbit IgG