CST, 85852S, Synapsin-2 (D6S9C) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying SYN2. Validated for Western Blotting,Immunoprecipitation,Immunofluorescence (Frozen). Available in 2 sizes. Highly specific and rigorously validated in-house, Synapsin-2 (D6S9C) Rabbit Monoclonal Antibody (CST #85852) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunofluorescence (Frozen): 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Frozen) Specificity / Sensitivity Synapsin-2 (D6S9C) Rabbit Monoclonal Antibody recognizes endogenous levels of total synapsin-2 protein Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly503 of human synapsin-2 protein. Background Synapsins, a group of at least five related members (synapsins Ia, Ib, IIa, IIb, and IIIa), are abundant brain proteins essential for regulating neurotransmitter release (1,2). All synapsins contain a short amino-terminal domain that is highly conserved and phosphorylated by PKA or CaM kinase I (1). Phosphorylation of the synapsin amino-terminal domain at Ser9 inhibits its binding to phospholipids and dissociates synapsins from synaptic vesicles (2). Synapsin proteins help control release of neurotransmitters by tethering clusters of synaptic vesicles to the actin cytoskeleton at pre-synaptic terminals (3). As might be expected given the role these proteins play in neuronal cell function, mutations in the corresponding synapsin genes have been examined for association with neurological disorders. Mutations in the corresponding gene tentatively implicate synapsin-2 in susceptibility to schizophrenia, bipolar disorder, and autism spectrum disorders (4-6). Alternate Names SYN2; Synapsin II; Synapsin-2; SYNII; SYNIIa; SYNIIb Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 77 Source/Isotype: Rabbit IgG