CST, 8586S, Dopamine beta-Hydroxylase (DBH) Antibody

Polyclonal Antibody for studying DBH. Validated for Western Blotting. Highly specific and rigorously validated in-house, Dopamine beta-Hydroxylase (DBH) Antibody (CST #8586) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Dopamine beta-Hydroxylase (DBH) Antibody recognizes endogenous levels of total DBH protein. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala211 of human dopamine β-hydroxylase protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Dopamine β-Hydroxylase (DBH) is an enzyme of the copper type II ascorbate-dependent mono-oxygenase family. This enzyme forms homotetramers composed of two noncovalently bound disulfide-linked dimers and is found as both membrane-associated and soluble forms (1-3). The soluble form is present in the lumen of secretory granules (4) and is released from cells by exocytosis (5). DBH converts dopamine to noradrenaline (6). Deficiency in this enzyme causes a rare disease characterized by a complete absence of noradrenaline and adrenaline in plasma together with increased plasma dopamine levels (7). Orthostatic hypotension, the main symptom of DBH deficiency, can be alleviated by administration of dihydroxyphenylserine, a synthetic precursor of noradrenaline (8). Alternate Names DBH; DBM; Dopamine beta-hydroxylase; dopamine beta-hydroxylase (dopamine beta-monooxygenase); Dopamine beta-monooxygenase; DOPO; ORTHYP1; Soluble dopamine beta-hydroxylase Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 75-80 SOURCE: Rabbit