CST, 8588T, hnRNP Q/R (D18B2) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying hnRNP R/hnRNP Q. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, hnRNP Q/R (D18B2) Rabbit Monoclonal Antibody (CST #8588) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity hnRNP Q/R (D18B2) Rabbit Monoclonal Antibody recognizes endogenous levels of total hnRNP Q and hnRNP R proteins. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys371 of human hnRNP Q protein. Background Heterogeneous nuclear ribonucleoprotein Q and R belong to a family of hnRNP proteins that are involved in RNA binding, RNA biosynthesis, and mRNA transport from the nucleus to the cytoplasm (1-3). These two proteins are encoded by different genes but have 83% homology. hnRNP Q has three alternative splice variants (hnRNP Q1-3) (1-3). Methylation of carboxy-terminal arginine residues is required for nuclear localization (4). hnRNP Q binds to AU-rich mRNA in conjunction with AUF1 and regulates mRNA decay (5). hnRNP Q isoforms play a crucial role in mediating nuclear function of survival of motor neuron (SMN) complex (6,7) and modulating RNA biosynthesis and hepatitis C virus replication (8). hnRNP R was identified recently and its function is still under investigation (9), however hnRNP R does not duplicate the biological function of hnRNP Q. Both hnRNP Q and R are present in cytoplasmic mRNP granules containing untranslated mRNAs (10) and both interact with SMN (6). Alternate Names dJ3J17.2; FLJ25714; FLJ31626; Glycine- and tyrosine-rich RNA-binding protein; GRY-RBP; GRYRBP; Heterogeneous nuclear ribonucleoprotein Q; Heterogeneous nuclear ribonucleoprotein R; hnRNP Q; hnRNP R; hnRNP-Q; hnRNP-R; HNRNPQ; HNRNPR; HNRPQ; HNRPQ1; HNRPR; NS1-associated protein 1; NSAP1; PP68; synaptotagmin binding cytoplasmic RNA interacting protein; synaptotagmin binding, cytoplasmic RNA interacting protein; Synaptotagmin-binding, cytoplasmic RNA-interacting protein; SYNCRIP Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 65-82 Source/Isotype: Rabbit IgG