CST, 85891T, ZFP36L2 (E4M9Z) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying ZFP36L2/BRF2. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, ZFP36L2 (E4M9Z) Rabbit Monoclonal Antibody (CST #85891) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity ZFP36L2 (E4M9Z) Rabbit Monoclonal Antibody recognizes endogenous levels of total ZFP36L2 protein. This antibody does not cross-react with ZFP36L1 protein. Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly237 of human ZFP36L2 protein. Background ZFP36L1, also known as butyrate response factor-1 (BRF1), and ZFP36L2, also known as butyrate response factor-2 (BRF2), both belong to the TIS11 family of CCCH zinc-finger proteins (1). This family of proteins, which also includes tristetraprolin (TTP), bind to AU-rich elements (AREs) found in the 3'-untranslated regions of mRNAs and promote deadenylation and rapid degradation by the exosome (2,3). These proteins play a critical role in cell growth control by regulating the mRNA turnover of multiple cytokines, growth factors, and cell cycle regulators, including GM-CSF, TNFα, IL-2, IL-3, and IL-6 (4,5). Deregulated ARE-mRNA stability can contribute to both inflammation and oncogenic transformation (6-8). Insulin-induced stabilization of ARE-containing transcripts is mediated by Akt/PKB phosphorylation of ZFP36L1 at Ser92, which results in binding by 14-3-3 protein and inactivation of ZFP36L1 (9). ZFP36L1 and L2 have also been shown to promote cell quiescence in developing B lymphocytes, promoting VDJ recombination (10). ZFP36L2 plays a role in oocyte maturation, ovulation, and female fertility (11,12). It also has been shown to suppress regulatory T cell function through downregulation of Helios (13). Alternate Names BRF2; Butyrate response factor 2; butyrate response factor 2 (EGF-response factor 2); EGF-response factor 2; ERF-2; ERF2; mRNA decay activator protein ZFP36L2; Protein TIS11D; RNF162C; TIS11D; TISD; TPA-induced sequence 11d; ZFP36 ring finger protein like 2; ZFP36-like 2; ZFP36L2; zinc finger protein 36, C3H type-like 1; zinc finger protein 36, C3H type-like 2; Zinc finger protein 36, C3H1 type-like 2; zinc finger protein, C3H type, 36-like 2 Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 60 Source/Isotype: Rabbit IgG