CST, 85925SF, GAPDH (14C10) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying GAPDH. Validated for WB,IHC,IF,F,ELISA+. Highly specific and rigorously validated in-house, GAPDH (14C10) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #85925) is ready to ship. Product Usage Information This product is the carrier free version of product #2118. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 2118 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity GAPDH (14C10) Rabbit Monoclonal Antibody (BSA and Azide Free) detects endogenous levels of total GAPDH protein. Species Reactivity: Human, Mouse, Rat, Monkey, Bovine, Pig Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys260 of human GAPDH protein. Background Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. Though differentially expressed from tissue to tissue (1), GAPDH is thought to be a constitutively expressed housekeeping protein. For this reason, GAPDH mRNA and protein levels are often measured as controls in experiments quantifying specific changes in expression of other targets. Recent work has elucidated roles for GAPDH in apoptosis (2), gene expression (3), and nuclear transport (4). GAPDH may also play a role in neurodegenerative pathologies such as Huntington and Alzheimer's diseases (4,5). Alternate Names aging-associated gene 9 protein; epididymis secretory sperm binding protein Li 162eP; G3P; G3PD; GAPD; GAPDH; glyceraldehyde 3-phosphate dehydrogenase; Glyceraldehyde-3-phosphate dehydrogenase; HEL-S-162eP; MGC88685; OCAS, p38 component; Oct1 coactivator in S phase, 38 Kd component; Peptidyl-cysteine S-nitrosylase GAPDH Specification REACTIVITY: H M R Mk B Pg SENSITIVITY: Endogenous MW (kDa): 37 Source/Isotype: Rabbit IgG