CST, 8592S, Cleaved Caspase-8 (Asp387) (D5B2) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Casp8 (Asp387) cleaved mouse. Validated for WB,IP,IF,F. Available in 2 sizes. Highly specific and rigorously validated in-house, Cleaved Caspase-8 (Asp387) (D5B2) Rabbit Monoclonal Antibody (CST #8592) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Immunofluorescence (Immunocytochemistry): 1:400 - 1:1600 Flow Cytometry (Fixed/Permeabilized): 1:400 - 1:1600 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 97756 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity Cleaved Caspase-8 (Asp387) (D5B2) Rabbit Monoclonal Antibody recognizes endogenous levels of mouse caspase-8 only when cleaved at Asp387. In addition to the p18 subunit, this antibody also detects cleavage products containing the pro-domain with the p18 subunit (p43). Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp387 of mouse caspase-8 protein. Background Apoptosis induced through the CD95 receptor (Fas/APO-1) and tumor necrosis factor receptor 1 (TNFR1) activates caspase-8 and leads to the release of the caspase-8 active fragments, p18 and p10 (1-3). Activated caspase-8 cleaves and activates downstream effector caspases such as caspase-1, -3, -6, and -7. Caspase-3 ultimately elicits the morphological hallmarks of apoptosis, including DNA fragmentation and cell shrinkage. Alternate Names Casp; CASP-8; Casp8; caspase 8; Caspase-8; Caspase-8 subunit p10; Caspase-8 subunit p18; caspase8; Fas-linked ICE-like protease; FLI; FLICE; MAC; MACH; Mch5 Specification REACTIVITY: M SENSITIVITY: Endogenous MW (kDa): 18, 43 Source/Isotype: Rabbit IgG