CST, 85934T, Glutaminase-2/GLS2 (E9C7V) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying GLS2. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Glutaminase-2/GLS2 (E9C7V) Rabbit Monoclonal Antibody (CST #85934) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Glutaminase-2/GLS2 (E9C7V) Rabbit Monoclonal Antibody recognizes endogenous levels of total glutaminase-2/GLS2 protein. This antibody does not cross-react with glutaminase-1/GLS1 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human glutaminase-2/GLS2 protein. Background Glutaminase catalyzes the conversion of glutamine to glutamate, the first and rate-limiting step of glutaminolysis (1). Both kidney-type glutaminase (GLS1) and liver-type glutaminase (GLS2) are found in mammals (2). GLS1-mediated glutathione synthesis plays an essential role in redox homeostasis and contributes to increased survival of postimplantation bone cells preconditioned to the hypoxic and ischemic environment in the bone defect site (3). In addition, - -mutant LUAD ( -mutant lung adenocarcinoma) tumors are dependent on increased glutaminolysis (1). Furthermore, recent studies showed higher glutaminolysis and glucose production from glutamine in human primary hepatocytes with gain-of-function missense mutations (4). These findings suggest GLS1 and GLS2 as potential targets in the therapy of bone regeneration and in the treatments of diseases such as cancer and hyperglycemia, respectively (1,3,4). Alternate Names breast cell glutaminase; GA; GLS; GLS2; GLSL; glutaminase 2; glutaminase 2 (liver, mitochondrial); glutaminase GA; glutaminase I; Glutaminase liver isoform, mitochondrial; hLGA; L-glutaminase; L-glutamine amidohydrolase; LGA; MGC71567; phosphate-activated glutaminase; phosphate-dependent glutaminase Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 60 Source/Isotype: Rabbit IgG