CST, 85995S, LAMP1 (D2D11) Rabbit Monoclonal Antibody (PE Conjugate)

Monoclonal Antibody for studying LAMP1. Validated for Flow Cytometry (Fixed/Permeabilized). Highly specific and rigorously validated in-house, LAMP1 (D2D11) Rabbit Monoclonal Antibody (PE Conjugate) (CST #85995) is ready to ship. Product Usage Information Flow Cytometry (Fixed/Permeabilized): 1:50 Storage Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze. Protocol Available protocols: Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity LAMP1 (D2D11) Rabbit Monoclonal Antibody (PE Conjugate) recognizes endogenous levels of total LAMP1 protein. Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a recombinant protein fragment of human LAMP1 protein. Background Lysosome-associated membrane protein 1 and 2 (LAMP1 and LAMP2) are two abundant lysosomal membrane proteins (1,2). Both are transmembrane proteins and are heavily glycosylated at the amino-terminal luminal side of the lysosomal inner leaflet, which protects the proteins from proteolysis (3). The carboxy terminus of LAMP1 is exposed to the cytoplasm and contains a tyrosine sorting motif that targets LAMP to lysosomal membranes (4). LAMP1 and LAMP2 are 37% homologous in their protein sequences. Both LAMP1 and LAMP2 are involved in regulating lysosomal motility during lysosome-phagosome fusion and cholesterol trafficking (5,6). Alternate Names CD107 antigen-like family member A; CD107a; LAMP-1; LAMP1; LAMPA; LGP120; lysosomal associated membrane protein 1; lysosomal-associated membrane protein 1; Lysosome-associated membrane glycoprotein 1; Lysosome-associated membrane protein 1 Specification REACTIVITY: H Mk SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG